amp activated protein kinase α ampkα  (Cell Signaling Technology Inc)

Journal: Endocrinology and Metabolism

Article Title: Effects of Progranulin Deficiency on Inflammation and Fibrosis in the Kidneys and Liver of Diabetic Mice Fed a High-Fat Diet

doi: 10.3803/EnM.2025.2339

Figure Lengend Snippet: Sodium-glucose co-transporter-2 inhibitor- and progranulin knockout-induced autophagy via the mechanistic target of rapamycin complex 1 pathway in the kidneys of each group. Western blot analysis of the protein levels of (A) phosphorylated AMP-activated protein kinase (p-AMPK) and AMPK, (B) p-Akt and Akt, and (C) p-S6 and S6 in the kidneys. Comparisons among groups were performed with one-way analysis of variance followed by Tukey tests. Data are expressed as mean±standard deviation. GAPDH, glyceraldehyde 3-phosphate dehydrogenase; WT, wild-type; DM, diabetes mellitus; Tofo, tofogliflozin; KO, knockout. a P <0.05, b P <0.01 vs. WT-DM; c P <0.05 vs. WT-Tofo.

Article Snippet: Membranes were blocked with a blocking reagent (0.1% Tween 20 and 5% bovine serum albumin in Tris-buffered saline) for 1 hour and subsequently incubated overnight at 4°C with the following primary antibodies (1:1,000): phospho-S6 (#2211; Cell Signaling Technology, Danvers, MA, USA), S6 (#2217; Cell Signaling Technology), phosphorylated AMP-activated protein kinase (p-AMPK) (#2531; Cell Signaling Technology), AMPK (#2532; Cell Signaling Technology), phospho-Akt (#4060; Cell Signaling Technology), Akt (#4691; Cell Signaling Technology), peroxisome proliferator-activated receptor α (PPARα) (#SC-398394; Santa Cruz Biotechnology, Dallas, TX, USA), and PPARγ (#2435; Cell Signaling Technology).

Techniques: Knock-Out, Western Blot, Standard Deviation

Journal: Endocrinology and Metabolism

Article Title: Effects of Progranulin Deficiency on Inflammation and Fibrosis in the Kidneys and Liver of Diabetic Mice Fed a High-Fat Diet

doi: 10.3803/EnM.2025.2339

Figure Lengend Snippet: Sodium-glucose co-transporter-2 inhibitor- and progranulin knockout-induced autophagy via the mechanistic target of rapamycin complex 1 pathway in the livers of each group. Western blot analysis of the protein levels of (A) phosphorylated AMP-activated protein kinase (p-AMPK) and AMPK, (B) p-Akt and Akt, and (C) p-S6 and S6 in the liver. Comparisons among groups were performed with one-way analysis of variance followed by Tukey tests. Data are expressed as mean±standard deviation. GAPDH, glyceraldehyde 3-phosphate dehydrogenase; WT, wild-type; DM, diabetes mellitus; Tofo, tofogliflozin; KO, knockout. a P <0.05.

Article Snippet: Membranes were blocked with a blocking reagent (0.1% Tween 20 and 5% bovine serum albumin in Tris-buffered saline) for 1 hour and subsequently incubated overnight at 4°C with the following primary antibodies (1:1,000): phospho-S6 (#2211; Cell Signaling Technology, Danvers, MA, USA), S6 (#2217; Cell Signaling Technology), phosphorylated AMP-activated protein kinase (p-AMPK) (#2531; Cell Signaling Technology), AMPK (#2532; Cell Signaling Technology), phospho-Akt (#4060; Cell Signaling Technology), Akt (#4691; Cell Signaling Technology), peroxisome proliferator-activated receptor α (PPARα) (#SC-398394; Santa Cruz Biotechnology, Dallas, TX, USA), and PPARγ (#2435; Cell Signaling Technology).

Techniques: Knock-Out, Western Blot, Standard Deviation

Journal: Journal of Ovarian Research

Article Title: Yangjingzhongyu Decoction regulates primordial follicle initiation via LncRNA-Smad1/AMPK dual pathways in diminished ovarian reserve

doi: 10.1186/s13048-025-01955-1

Figure Lengend Snippet: Proposed mechanism of YJZYD in regulating the primordial follicle initiation rate and microenvironment. YJZYD inhibits LTCONS-00011173 expression, leading to enhanced Smad1 phosphorylation. This activates two downstream pathways: (1) AMPK/SIRT1/PGC-1α pathway promoting mitochondrial biogenesis and antioxidant function (increased SOD, ATP; decreased MDA). (2) Modulation of follicular regulatory factors (decreased PTEN, MST; increased AMH, GDF9) to balance primordial follicle initiation and growth. Solid arrows indicate direct activation; dashed arrows denote indirect regulation or material transport

Article Snippet: Protein bands were analyzed using an electrochemilluminescence (ECL) chemil uminescence kit and the bands intensity was quantified using the GIS1000 Imaging system with the following primary antibodies: Smad1 polyclonal antibody (10429-1-AP, 1:5000, Proteintech, USA), Anti-Mullerian hormone (AMH) polyclonal antibody (14461-1-AP, 1:5000, Proteintech, USA), phosphate and tensin homolog (PTEN) polyclonal antibody (10047-1-AP, 1:5000, Proteintech, USA), AMP-activated protein kinase (AMPK) polyclonal antibody (10929-2-AP, 1:5000, proteintech, Wuhan, China) and SIRT1 polyclonal antibody (A00018-1, 1:1000, Boster, Wuhan, China).

Techniques: Expressing, Phospho-proteomics, Activation Assay

Journal: Journal of Ovarian Research

Article Title: Yangjingzhongyu Decoction regulates primordial follicle initiation via LncRNA-Smad1/AMPK dual pathways in diminished ovarian reserve

doi: 10.1186/s13048-025-01955-1

Figure Lengend Snippet: Molecular docking of YJZYD monomers. A Heatmap of binding energies. B - H . 2D interaction diagrams: ( B ) AMPK-Curcumin, ( C ) AMPK-m-Cresol, ( D ) SIRT1-Apigenin, ( E ) SIRT1-Curcumin, ( F ) SIRT1-m-Cresol, ( G ) MAP3K7-Apigenin, ( H ) MAP3K7-m-Cresol. I Docking energy legend. Docking scores: Strong binding: < -8 kcal/mol; Moderate: < -5 kcal/mol

Article Snippet: Protein bands were analyzed using an electrochemilluminescence (ECL) chemil uminescence kit and the bands intensity was quantified using the GIS1000 Imaging system with the following primary antibodies: Smad1 polyclonal antibody (10429-1-AP, 1:5000, Proteintech, USA), Anti-Mullerian hormone (AMH) polyclonal antibody (14461-1-AP, 1:5000, Proteintech, USA), phosphate and tensin homolog (PTEN) polyclonal antibody (10047-1-AP, 1:5000, Proteintech, USA), AMP-activated protein kinase (AMPK) polyclonal antibody (10929-2-AP, 1:5000, proteintech, Wuhan, China) and SIRT1 polyclonal antibody (A00018-1, 1:1000, Boster, Wuhan, China).

Techniques: Binding Assay

Journal: Journal of Ovarian Research

Article Title: Yangjingzhongyu Decoction regulates primordial follicle initiation via LncRNA-Smad1/AMPK dual pathways in diminished ovarian reserve

doi: 10.1186/s13048-025-01955-1

Figure Lengend Snippet: YJZYD enhances mitochondrial function and antioxidant capacity. A AMPK/SIRT1/PGC-1α mRNA expression. B - C Corresponding protein expression and immunoblots. D - F SOD, MDA, and ATP levels. Statistical analysis ( n = 3): Model vs. Control: * p < 0.05; YJZYD vs. Model: # p < 0.05. MRNA compared to β-actin, protein compared to GAPDH

Article Snippet: Protein bands were analyzed using an electrochemilluminescence (ECL) chemil uminescence kit and the bands intensity was quantified using the GIS1000 Imaging system with the following primary antibodies: Smad1 polyclonal antibody (10429-1-AP, 1:5000, Proteintech, USA), Anti-Mullerian hormone (AMH) polyclonal antibody (14461-1-AP, 1:5000, Proteintech, USA), phosphate and tensin homolog (PTEN) polyclonal antibody (10047-1-AP, 1:5000, Proteintech, USA), AMP-activated protein kinase (AMPK) polyclonal antibody (10929-2-AP, 1:5000, proteintech, Wuhan, China) and SIRT1 polyclonal antibody (A00018-1, 1:1000, Boster, Wuhan, China).

Techniques: Expressing, Western Blot, Control